By José M. Guisán

The second one version of Immobilization of Enzymes and Cells serves as an replace in addition to a praise to the 1st version. This quantity now comprises easy protocols for the immobilization of enzymes and cells that may be beneficial for program at commercial scale, novel protocols for immobilization sooner or later, and new chemical reactors in a position to triumph over the restrictions of a few immobilized derivatives.

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Preparation and properties of an amyloglucosidase adsorbate. Biotechnol. Bioeng. 18, 1589–1604. , and Javed, A. (1982) Enzyme immobilization on trityl-agarose: Reusability of both matrix and enzyme. Bioechnol. Bioeng. 24, 1221–1224. Yon, R. (1974) Enzyme purification by hydrophobic chromatography: an alternative approach illustrated in the purification of aspartate transcarbamoylase from wheat germ. Biochem. J. 137, 127–130. , and Butler, L. (1979) Hydrophobic esters of cellulose: properties and applications in biochemical technology.

1971) Changes in conformation of insolubilized trypsin and chymotrypsin, followed by fluorescence. Biochemistry 10, 4661–4669. T. (1976) Exoenzymic activity of alpha-amylase immobilized on a phenol-formaldehyde resin Carbohydr. Res. 48, 239–244. Guisán, J. , et al. (1997) Immobilization of enzymes acting on macromolecular substrates.. In: Immobilization of Enzymes and Cells, (Bickerstaff, G. ), Humana Press, Totowa, NJ, pp. 261–275. CLEAS 31 3 Cross-Linked Enzyme Aggregates Roger A. Sheldon, Rob Schoevaart, and Luuk M.

Enzyme Microb. Technol. 10, 375–382. 26. Wilchek, M. and Miron, T. (1982) A spectrophotometric assay for soluble and immobilized N-hydroxysuccinimide esters. Anal. Biochem. 126, 433–435. 27. , and Svec, F. (1982) The activation of hydroxy groups of carriers with 4-nitrophenyl and N-hydroxysuccinimidyl chloroformates. Biotechnol. Bioeng. 24, 487–493. 28. , and Cuatrecasas, P. (1974) Topics in the methodology of substitution reactions with agarose. B. and Wilchek, M. ), Academic Press, New York, NY, pp.

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