By Harris Busch (Eds.)
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Extra resources for The Cell Nucleus. Volume 2
Example text
Other types of inversions, such as symmetrical pericentrics and paracentric inversions (in which the centromere is not involved), could only be postulated before 1970. To date they have not yet been proven in humans, but we predict they will be described within a few years using the new chromosome-banding methods. 5 . AUTOSOMAL DELETIONS a. Constitutional Autosomal Deletions. Dogmatism in science usually is the handmaid of ignorance. Thus it was dictum prior to 1963 that 2. Differential Staining Techniques 39 loss of autosomal chromosome material was lethal.
70, 263. 2 The Human Cell Nucleus: Quinacrine and Other Differential Stains in the Study of Chromatin and Chromosomes Frederick Hecht, Herman E . Wyandt, a n d R. E l l e n H e a t h M a g e n i s I. Perspectives on Human and Medical Cytogenetics II. Human Chromosome Identification A. Before Banding B. Classification of Banding Techniques C. Methodology of Banding D. Characterization of Human Chromosomes by Banding E. Other Useful Techniques III. Human Chromosome Polymorphisms A. By Conventional Giemsa Staining B.
Barski et al. ( 1961 ) used two mouse cell lines differing in several marker chromosomes for their hybridization experiments. In the resulting (heterokaryon) cells not only did the chromosome number increase to 24 FRANCES E . ARRIGHI η Ν 1>' ΧΥ M NI Ii 6* ίί $s A A Aft ft* ft* iU si Aft Αι» AS *ft UM Fig. 7 G-band pattern of a canyon mouse, Peromyscus crinitus. Note all chromosome pairs can be distinguished. ( Courtesy of Dr. Sen Pathak. ) the sum of the two original numbers, but also the marker chromosomes of both lines were present.
