By Eric J. Hall
Now in complete colour, this lavishly illustrated re-creation is replete with tables and figures that underscore crucial thoughts. each one bankruptcy concludes with a "summary of pertinent conclusions" to facilitate speedy assessment and support readers retain important information.
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Example text
Indeed, the one does not exclude the other because some portions of the DNA may be intimately involved with the membrane during some portions of the cell cycle. The evidence implicating the chromosomes, specifically the DNA, as the primary target for radiation-induced lethality may be summarized as follows: 1. Cells are killed by radioactive tritiated thymidine incorporated into the DNA. The radiation dose results from short-range ␣particles and is therefore very localized. 2. indd 40 place of thymidine if substituted in cell culture growth medium.
Lymphocytes in the blood sample are stimulated to divide with a mitogen such as phytohemagglutinin and are arrested at metaphase, and the incidence of rings and dicentrics is scored. The dose can be estimated by comparison with in vitro cultures exposed to known doses. 20 shows a doseresponse curve for aberrations in human lymphocytes produced by ␥-rays. The data are fitted by a linear-quadratic relationship, as would be expected, because rings and dicentrics result from the interaction of two chromosome breaks, as previously described.
This loss of DNA may include the loss of a suppressor gene and lead to a malignant change. See Chapter 10 on radiation carcinogenesis. Matrix attachment sites Prereplication interphase chromosome Nuclear membrane Two radiation-induced breaks occur in the same arm of a chromosome Acentric ring A deletion occurs as an acentric ring; lost at a subsequent mitosis (such as dicentrics) die in attempting a subsequent mitosis, but those that sustain a symmetric nonlethal aberration (such as a translocation) survive and pass on the aberration to their progeny.